ON THE INFLUENCE OF THE BIORESONANCE TREATMENT ON TUMOR DEVELOPMENT IN EXPERIMENTAL ANIMALS

G. Lednyiczky1, O. Lakiza2, S. Shandrenko2, O. Zhalko-Tytarenko1, T. Buzasi1
1. Hippocampus Research Facilities, H-1092 Budapest, Ráday utca 8., Hungary, Tel:+361.299.0200, Fax: +361.299.0836, e-mail: hippocam@hippocampus-brt.com
2. Inst. of the Experimental Pathology, Oncology, and Radiobiology of the Natl. Acad. of Sci. of the Ukraine, Kiev, Ukraine

Abstract
Every living cell produces a specific electronmagnetic field (EMF). The EMF can be accumulated modulated and amplificated by the BICOM instrument. The effect of these treatments were examined in different animal tumor models. In some cases (e.g., B16 melanoma) the BICOM’s bioresonance therapy (BRT) inhibited the primary tumor growth and the development of metastases. The tumor growth of Heren carcinoma and Ehrlich carcinoma were not changed by the BRT. Some changes in the signals of paramagnetic metasbolites and free radicals were observed. Further experiments need to explain the mechanism of BRT. This treatment method may be useful for cancer therapy in the future.

Introduction

The cells of different organisms are assumed [1] to receive information regularly from their environment. This process may require not only a chemical substances but biophysical signals. All biochemical processes generate a wide range of frequencies of endogenous EMFs which correspond to the processes’ characteristic relaxation times. Moreover, processes that occur at the biochemical level are then controlled by these fields or - say at the biophysical level. Any “biochemical disorder” sooner or later will affect the whole organism via the “reflection” and feedback from the biophysical level. Vice versa, biophysical level therapy will treat the organism as a whole by restoring the damaged links at the biochemical level rather than by only fixing a particular biochemical problem. The resonant intera
ctions between the endogenous electromagnetic fields of biological systems from subcellular structures, cells, organs, organisms to populations are usually referred to as bioresonant interactions. However, after the division, cells start acquiring positional information again. L. Wolpert [2] proposes that positional information is necessery during an organism’s development and differentiation.
M. Terzi [3] suggests that positional information determines molecular differentiation, the biosynthesis of different biochemical pathways. Positional information may play an important role in cell-cell interactions, communication and recognition [4]. According to this theory, development is a sequence of gene expressions under the control of positional information. Some authors suggest that positional information is transferred by bioactive substances [5] or by biophysical mechanisms via autowaves [6, 7]. Weak electromagnetic fields are shown to alter genome activity, namely - gene transcripts [8]. Electromagnetic fields also alter membrane Na, K-ATPase activity [9], Plasmids (cell-free Transcription/Translation Systems) were exposed to a magnetic field which resulted in enhanced gene expression. [10]. According to A. Gurwitch [4, 11], homeostasis is controlled by a special anisotropic field of cells which is coupled to vectorial subcellular organization and ordering of molecular constellations. N. Koltsov considers that every cell has electromagnetic fields which are induced by gradients of physical and chemical potentials within the cells. Some experiments deal with the changes in the state of tumor cells under the influence of endogenous EMF. Sheldrake [12] suggested the morphogenetic field to be similar to known physical fields that can have an electromagnetic feature [8].
We suppose that the discrepancies between a cell’s competence and positional information may cause the organism’s control of transformed cells to be lessened. Tumor incidence may be due to an inappropriate balance between proliferation and differentiation, resulting in over-proliferation. Cells of a malignant phenotype have all genes that regulate normal proliferation [3, 13]. That is why it is plausible to conclude that “ malignancy can be suppressed by inducing differentiation either with or without genetic changes in the malignant cells; this suppression does not have to restore all normal controls.” [14 - 16].
There may be various factors responsible for such an imbalance, however this imbalance may be restored by applying bioresonance therapy (BRT) [1, 13, 17-21]. Bioresonance is considered a general mechanism of maintaining the communication pathways within living matter via endogenous EMF interactions [18-20]. Although critics argue that bioresonance has no strict supporting theory, it has been successfully employed in medicine for the past two decades [13, 21-25]
Probably, the first attempts at treating cancer with electromagnetic fields (EMF) began in the 1930’s when Nikola Tesla and Georges Lakhovsky constructed the Multiple Wave Oscillator: a generator of life-associated frequencies from 750 kHz to 3 MHz and numerous harmonics, which may extend as far as 300 GHz. They reported several successful applications of the device in cancer treatment and various metabolic disorders in plant, animal and human patients [26]. Another therapeutic device from that period was invented and applied for cancer treatment by Wilhelm Reich [27, 28]. Unlike Lakhovsky’s Oscillator, it was not generating any EMFs but rather accumulating naturally occurring electromagnetic fields, which he termed “orgone energy”. Both devices were intended to “harmonize” the endogenous electromagnetic oscillations of patients with the environmental EMFs by employing a wide range of EM frequencies from very high EMF frequencies to extremely low frequencies (including Schumann waves [29]) with the Multiple Wave Oscillator and the Orgone Accumulator, respectively. These works, as well as further achievements in various EMF applications in healing processes (starting from the late 50’s [17]), made it possible to introduce the notion of bioresonance therapy (BRT) [1].
ESR spectroscopy is advantageous in this respect since during tumor development, all free radical sites and paramagnetic metal complexes alter substantially in quantity. These changes may be due to an excess production or metabolism of paramagnetic complexes as well as conformational alterations.
In order to estimate the non-specific response of animals with tumors to BRT, the content of various molecular weight fractions of the animal’s blood serum was measured by high performance liquid chromatography (HPLC).
In vivo studies of tumor development by the interference from various metabolic changes in a tumor-bearing organism. In order to determine the differences in the metabolic response to different types of tumors: lung metastasizing melanoma B16, Heren carcinoma (both solid tumors) and ascitic Ehrlich carcinoma were studied.

To elucidate possible mechanisms of the influence of endogenous EMF on tumor cells, the study was divided into the following steps:

1. Tumor development in mice after BRT of inoculated cells in vitro;
a) weight of primary tumor;
b) quantity and volume of metastases;
c) weight of immunocompetent organs;
2. Tumor development in mice after BRT of tumor-bearing animals.
a) weight of primary tumor;
b) quantity and volume of metastases;
c) weight of immunocompetent organs